The extraction of high‐quality RNA from plant tissues is a critical prerequisite for gene expression studies and other molecular analyses. Over the past decades, researchers have refined methods to ...
Van Andel Institute scientists have developed a new extraction protocol for RNA-seq and metabolomic analysis, offering a more complete picture of cellular activity than either technique on its own.
Figure 1: RNA-seq data uses short reads of mRNA which is free of intronic non-coding DNA. These reads must then be aligned back to the reference genome. Credit: Technology Networks. Early RNA-seq ...
To obtain high-quality, purified nucleic acids, scientists can use several different methods, including phenol-chloroform extraction, cesium chloride/ethidium bromide (CsCl/EtBr) gradient ...
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