RT-qPCR stands for quantitative reverse transcription polymerase chain reaction, the “RT” not to be mistaken for “real time”. 2 Unlike qPCR that uses a DNA template, the starting material for RT-qPCR ...
qPCR Analysis, How a qPCR Machine Works and qPCR Protocol In this article, we will consider how qPCR works, experimental requirements, data analysis and applications of the technique.