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Restriction Digest Analysis
8:02
YouTubeAddgene
Restriction Digest Analysis
In this video, we will digest a plasmid of known identity with restriction enzymes, run the products on an agarose gel, and analyze the results. We’ll focus on identifying the bands produced under different conditions and use the results to make conclusions about the plasmid. Note: XbaI is effected by Dam methylation - see this blog post for ...
218.3K views13 Dec 2016
Restriction Digest Tutorial
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Understanding Restriction Enzymes in DNA: Sticky End vs. Blunt End Cuts
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